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Updated: Jan 17, 2026
Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
Published on: March 22, 2020
Quantitative Analysis of Glutathione Utilizing Highly Luminescent Gold Nanoclusters
Wen He1, Yanping Qu1, Shukai Wu1
1Qilu Institute of Technology, College of Biological and Chemical Engineering, Jinan, Shandong, China.
Abstract:
Glutathione (GSH), a pivotal regulator of cellular redox homeostasis, requires precise monitoring for clinical diagnostics. This work develops bovine serum albumin-templated gold nanoclusters (BSA-AuNCs) through in situ chloroauric acid reduction, exhibiting orange fluorescence (λem = 610 nm) with 35.8% quantum yield. Systematic evaluation of pH-dependent and time-resolved quenching behavior revealed optimal GSH detection at physiological pH 7.4 within 3 min. The nanozyme demonstrated linear responsivity from 10 nM to 3 μM (Limit of Detection, LOD = 8.7 nM) and validated applicability in biological matrices (urine/serum) with 98.0%-103.3% recovery rates. This photoluminescent platform enables reliable GSH biomarker quantification, advancing point-of-care testing for oxidative stress disorders.
