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Rapid Identification of Pathogens01:25

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MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

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Biosensor Strategies for Rapid African Swine Fever Virus Detection.

Chelsie Boodoo1, Evangelyn C Alocilja1

  • 1Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, Michigan 48824, United States.

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|September 22, 2025
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Summary

Rapid African swine fever (ASF) detection is crucial for global pork safety. This review highlights the need for improved biosensor technology, focusing on accuracy, scalability, and cost-effectiveness to combat this lethal disease.

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Area of Science:

  • Veterinary diagnostics
  • Biosensor technology
  • Animal disease surveillance

Background:

  • African swine fever (ASF) poses a significant threat to global pig populations and the pork industry.
  • Current diagnostic methods are accurate but time-consuming and require laboratory settings.
  • There is a critical need for rapid, pen-side diagnostic alternatives for ASF.

Purpose of the Study:

  • To review and analyze existing African swine fever virus (ASFV) biosensors.
  • To identify limitations and challenges in current ASFV biosensor development.
  • To propose strategies for advancing ASFV biosensor technology towards commercial viability.

Main Methods:

  • Systematic review of 41 ASFV biosensors reported between 2014 and 2025.
  • Categorization of biosensors by biological receptor (antibody, enzyme, nucleic acid) and transduction mechanism (optical, mass-based, electrochemical).
  • Evaluation of reported limits of detection, assay times, sensitivity, and specificity.

Main Results:

  • ASFV biosensors show promising detection limits (5 copies/µL to 0.5 ng/mL) and rapid assay times (5-45 min).
  • Significant deficits persist in cross-reactivity analysis and environmental stress testing for many biosensors.
  • Current research highlights a gap between proof-of-concept and commercially viable ASF diagnostic tools.

Conclusions:

  • Harmonized testing conditions and comparative studies are essential for ASFV biosensor validation.
  • Learning from advanced viral pathogen platforms can guide practical design strategies for ASFV biosensors.
  • Advancing ASFV biosensor technology is vital for effective outbreak mitigation and safeguarding global pork production.