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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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A Practical Guide to Phylogenetics for Nonexperts
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Recombination and phylogenetic inference.

Bruce Rannala1

  • 1Department of Evolution and Ecology, University of California Davis, Davis, CA 95616, United States.

Evolutionary Journal of the Linnean Society
|September 22, 2025
PubMed
Summary
This summary is machine-generated.

Recombination significantly impacts phylogenetic tree inference. Species tree methods are more robust to recombination than concatenation methods, suggesting recombination detection may not always be necessary.

Keywords:
ancestral recombination graphcoalescentrecombinationspecies tree inference

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Area of Science:

  • Evolutionary biology
  • Phylogenetics
  • Population genetics

Background:

  • Recombination presents challenges for inferring evolutionary history.
  • Two common methods, concatenation and species tree inference, handle recombination differently.

Purpose of the Study:

  • To evaluate the impact of recombination on phylogenetic inference methods.
  • To assess strategies for addressing recombination in phylogenetics.

Main Methods:

  • Reviewed statistical robustness of methods ignoring recombination.
  • Examined methods accommodating recombination by identifying recombinant regions.
  • Considered methods inferring varying gene trees across the genome.

Main Results:

  • Recombination is more detrimental to concatenation methods than species tree inference.
  • Species tree methods show minimal impact on topology and divergence time estimates.
  • Eliminating recombinant loci may introduce bias in parameter estimates.

Conclusions:

  • Species tree inference methods are robust to recombination.
  • Recombination detection may be unnecessary for species tree inference.
  • Developing methods to combine varying gene trees with recombination remains a challenge.