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Updated: Jan 17, 2026
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Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
Published on: July 15, 2015
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Enhanced Kidney Targeting and Distribution of Tubuloids During Normothermic Perfusion
Enrique Montagud-Marrahi1,2,3, Adriana Rodriguez-Gonzalo2, Rubén López-Aladid2
1Kidney Transplant Unit, Nephrology and Kidney Transplantation Department, Hospital Clinic of Barcelona, Barcelona, Spain.
Summary
Human tubuloids show promise for kidney regeneration. Normothermic perfusion effectively delivers tubuloids for enhanced integration and potential treatment of kidney diseases.
Area of Science:
- Regenerative Medicine
- Nephrology
- Biotechnology
Background:
- Tubuloids are emerging tools for kidney disease modeling and regeneration.
- Limited data exists on their in vivo integration and regenerative capacity.
Purpose of the Study:
- To establish and compare human tubuloids from different protocols.
- To evaluate tubuloid delivery methods for kidney regeneration.
- To assess the potential of tubuloids in kidney repair and graft conditioning.
Main Methods:
- Established and characterized human tubuloids using two protocols: Crude and F4 (proximal tubular cells).
- Developed rat-derived tubuloids.
- Compared subcapsular injection versus normothermic perfusion for tubuloid delivery in rat and human kidney models.
Main Results:
- F4 tubuloids exhibited a higher differentiation state than Crude tubuloids.
- Normothermic perfusion was more efficient for tubuloid delivery compared to in vivo injection.
- Developed tubules were observed in host parenchyma one week and one month post-infusion.
Conclusions:
- Tubuloid infusion during normothermic perfusion is a viable strategy for kidney regeneration.
- This method enhances the translatability of kidney regenerative therapies.
- Potential applications include conditioning kidney grafts and treating kidney diseases.
