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A Single-Nucleus Transcriptomic Atlas of the Mouse Lumbar Spinal Cord: Functional Implications of Non-Coding RNAs
Pablo Ruiz-Amezcua1,2, Miguel Nieto Hernández1, Javier García Flores1
1Molecular Neuroprotection Group, Instituto de Investigación Sanitaria de Castilla-La Mancha, National Hospital for Paraplegics (SESCAM), 45071 Toledo, Spain.
This study presents a comprehensive single-nucleus RNA sequencing atlas of the adult mouse lumbar spinal cord, revealing 17 distinct neuronal subtypes. The research highlights the crucial role of non-coding RNAs in defining cell-specific signatures.
Area of Science:
- Neuroscience
- Genomics
- Transcriptomics
Background:
- The adult lumbar spinal cord is vital for movement and sensory processing.
- Previous studies had limited sample sizes for transcriptional analysis.
Purpose of the Study:
- To create a high-resolution transcriptomic atlas of the healthy adult mouse lumbar spinal cord.
- To investigate the contribution of non-coding RNAs to cell type specificity.
Main Methods:
- Integrative single-nucleus RNA sequencing (snRNA-seq) on over 86,000 nuclei from 16 samples.
- Harmonized computational pipeline for data analysis.
- Comparative transcriptomic analysis including coding and non-coding RNAs.
Main Results:
- Identification of all major spinal cell lineages.
- Resolution of 17 transcriptionally distinct neuronal subtypes.
- Demonstration that non-coding RNAs contribute significantly to cell type signatures.
Conclusions:
- The study provides an ncRNA-inclusive reference atlas for the adult spinal cord.
- This resource will facilitate future research on spinal cord plasticity, injury, and regeneration.
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