Related Experiment Video
Updated: Aug 6, 2026
09:17
Optimizing Extracellular Vesicle Delivery Using a Core-Sheath 3D-Bioprinted Scaffold for Chronic Wound Management
Published on: February 28, 2025
Extracellular Matrix-Based and Extracellular Matrix-Bioinspired Scaffolds for Extracellular Vesicle Delivery in
Nevena Cvetic1, Anđelka Zivaljevic1, Kristina Krstic1
1Department of Dentistry, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia.
Biotech (Basel (Switzerland))
|July 24, 2026
Summary
Scaffold-based delivery of extracellular vesicles (EVs) shows promise for dental pulp regeneration by enhancing tissue repair. However, further research is needed to standardize methods and prove clinical efficacy for regenerative endodontics.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Dental Research
Background:
- Vital pulp therapy aims to preserve tooth vitality but often achieves incomplete regeneration.
- Extracellular vesicles (EVs) show therapeutic potential for pulp regeneration due to their bioactive cargo.
- Clinical use of EVs is limited by rapid clearance and uncontrolled release.
Purpose of the Study:
- To critically review scaffold systems as delivery platforms for EVs in dental pulp regeneration.
- To compare the biological performance, methodological quality, and translational potential of these systems.
- To identify challenges and future directions for clinical implementation.
Main Methods:
- Narrative review of preclinical studies on scaffold-assisted EV delivery for dental pulp regeneration.
- Evaluation of studies based on EV source, isolation, characterization, scaffold properties, and outcome assessment.
- Assessment of biological outcomes including angiogenesis, odontogenesis, mineralization, and immunomodulation.
Main Results:
- Scaffold-assisted EV delivery consistently enhanced angiogenesis, odontogenic differentiation, mineralization, and immunomodulation in preclinical models.
- Significant heterogeneity exists in study methodologies, including EV source, scaffold composition, and outcome measures.
- Evidence remains preclinical, highlighting the need for standardization and further validation.
Conclusions:
- Scaffold-assisted EV delivery is a feasible strategy for regenerative endodontics.
- Challenges include standardization of EV production/characterization, scalable manufacturing, and regulatory approval.
- Well-designed translational and clinical studies are essential for routine implementation.