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Sucrose-Induced Transcriptomic Response in Ogataea polymorpha TBRC 4839 Reveals its Potential for Recombinant Protein
Somsak Likhitrattanapisal1, Chitwadee Phithakrotchanakoon1, Aekkachai Puseenam2
1Microbial Systems and Computational Biology Research Team, Thailand Bioresource Research Center, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Khlong Luang, Pathum Thani, Thailand.
The thermotolerant yeast Ogataea polymorpha efficiently produces proteins using inexpensive sugars. Its MAL promoter shows high potential for industrial protein production, especially with molasses.
Area of Science:
- Microbiology
- Molecular Biology
- Biotechnology
Background:
- Ogataea polymorpha is a thermotolerant yeast suitable for industrial protein expression.
- This yeast utilizes low-cost carbon sources like sucrose and molasses.
- Understanding its genomic and transcriptomic responses is key for optimizing production.
Purpose of the Study:
- To analyze the genome and transcriptome of O. polymorpha TBRC 4839.
- To identify genes and metabolic shifts induced by sucrose.
- To evaluate promoter efficiency for heterologous protein expression.
Main Methods:
- Genome sequencing and analysis of O. polymorpha TBRC 4839.
- Transcriptomic analysis under sucrose-induced conditions.
- Functional evaluation of gene promoters using fungal xylanase expression.
Main Results:
- The O. polymorpha TBRC 4839 genome is 8.9 Mbp with 5184 protein-coding genes.
- Sucrose induction shifts metabolism towards carbohydrate utilization and away from biosynthesis and cell division.
- The maltase (MAL) promoter demonstrated the highest efficiency for xylanase production, even with molasses.
Conclusions:
- O. polymorpha TBRC 4839 is a robust host for industrial protein production.
- The MAL promoter is highly effective for enhancing protein yields.
- This yeast and promoter combination offers a promising platform for cost-effective biomanufacturing.
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