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Updated: Jul 10, 2026
Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient
Published on: November 3, 2009
Serologic and molecular survey of Toxoplasma gondii in Baghdad Province, Iraq
Karrar Ali Mohammed Hasan Alsakini1, Hind H Al-Ammiri1, Mustafa Mohammed Touma1
1University of Baghdad, College of Veterinary Medicine, Microbiology Department, Baghdad, Iraq.
Background:
A prevalent contagious pathogenic parasite that can lead to major health issues is Toxoplasma gondii.
Aim:
The present study aimed to detect the parasitic immune response and the existence of genomic DNA in the blood of a T. gondii-positive equine.
Methods:
Thirty serum samples from horses suspected of having toxoplasmosis were collected from the Al-Rusafa neighborhood in Baghdad. To quantitatively investigate toxoplasma antibody levels in horse serum, an ELISA was used to evaluate immunoglobulin G (IgG) levels. Conventional (PCR) was used to identify T. gondii DNA.
Results:
The blood levels of IgG immunoglobulin in toxoplasma-infected horses differed significantly (p < 0.01) according to sex and age. Toxoplasma gondii-specific forward and reverse primers were generated using NCBI GenBank software. Toxoplasma genes were amplified using standard PCR. The proposed method can be used as a molecular diagnostic tool for detecting and comparing molecules using a ladder.
Conclusion:
The findings of this investigation were to ascertain whether the T. gondii genotype (UPRTF2 gene) is present. The size band of 443 bp DNA in the blood of toxoplasma-infected horses was confirmed using serological and molecular assessments. There were no statistically significant differences found by the Chi-square (χ2) test between the age groups or sexes of the seropositive and seronegative horses.
