Pasteurella multocida genome assembly of Indonesian local isolates using long-read Oxford Nanopore Technology...

Pasteurella multocida is a Gram-negative bacterium responsible for various diseases in animals, including hemorrhagic septicemia (HS). Oxford Nanopore Technologies' (ONT) platform streams the sequencing data immediately, allowing for on-the-fly analysis. This capability enables researchers to monitor experiments in real time and make prompt decisions, which is particularly beneficial for clinical diagnostics and outbreak surveillance.

This study presents the genome assembly of two P. multocida isolates using long-read sequencing technology provided by ONT.

This method involves passing DNA strands through nanopores and detecting changes in electrical current to determine nucleotide sequences. The long-read capability of ONT technology allows for the sequencing of extensive DNA fragments, which is particularly beneficial for resolving complex genomic regions and achieving high-contiguity genome assemblies.

The study generated high-quality single-contig genomes with sizes of 2.35 and 2.29 Mbp and completeness scores exceeding 99% (BUSCO). These assemblies provide valuable resources for functional analyses, enhancing the understanding of virulence factors and the pathogenesis of HS in Indonesian livestock.

This study successfully assembled the genomes of two P. multocida isolates from Lampung (2,952) and Kupang, Nusa Tenggara Timur, Indonesia, using Oxford Nanopore Technologies' long-read sequencing. The resulting single-contig genomes, measuring 2.35 and 2.29 Mbp, respectively, exhibited high completeness scores (BUSCO: >99%). These high-quality assemblies enhance genomic contiguity, providing valuable resources for functional analyses aimed at understanding the virulence factors and pathogenesis of HS in Indonesian livestock.