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Updated: Jan 17, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
Published on: March 14, 2013
Advancing Untargeted Metabolomics Through FT-ICR-MS: Challenges and Applications
Marta Sousa Silva1, Carlos Cordeiro1
1FT-ICR and Structural Mass Spectrometry Laboratory Biosystems and Integrative Sciences Institute (BioISI) Faculdade De Ciências Universidade De Lisboa Lisbon Portugal.
Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) offers unparalleled performance for untargeted metabolomic analysis. This review highlights advancements, applications, and future prospects of FT-ICR-MS in understanding complex biological systems.
Area of Science:
- Metabolomics
- Analytical Chemistry
- Biochemistry
Background:
- Metabolomics studies the complete set of small molecules (metabolites) in biological systems.
- It captures biochemical pathways and dynamic interactions, reflecting physiological and environmental states.
- Untargeted metabolomic analysis aims to identify and quantify all detectable metabolites.
Purpose of the Study:
- To review new developments in Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) for metabolomics.
- To discuss new methodologies and recent applications of FT-ICR-MS in the field.
- To address current challenges and future perspectives for FT-ICR-MS in metabolomic research.
Main Methods:
- Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) is the primary technology discussed.
- FT-ICR-MS provides extreme mass resolution and exceptional mass accuracy.
- This enables precise identification and differentiation of thousands of metabolites in complex samples.
Main Results:
- FT-ICR-MS allows for the simultaneous detection of thousands of compounds.
- It provides highly accurate molecular formulas based on exact mass and isotopic distribution.
- This technology is unmatched by other mass spectrometers for untargeted metabolomics.
Conclusions:
- FT-ICR-MS is the highest-performance technology for untargeted metabolomic analysis.
- Advancements in FT-ICR-MS continue to enhance its capabilities in biological research.
- The technology holds significant promise for future metabolomic studies and applications.
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