[Design and practice of undergraduate instrumental analysis experiment： analysis of polycyclic aromatic hydrocarbons...

A research-project-based open instrumental-analysis experiment was designed for undergraduate teaching led by the attributes of an environmental science major. In this experiment， porous monolithic fibers are in-situ synthesized from 9-vinylanthracene and styrene （as a monomer mixture） based on the molecular characteristics of studied polycyclic aromatic hydrocarbons （PAHs）， and then used as an extractant for solid-phase microextraction （SPME）. A method for reliably monitoring PAHs in environmental water samples was developed by combining SPME with high performance liquid chromatography （HPLC）. Students designed and constructed a complete experimental instrumental-analysis process， which included preparing and characterizing the monolith fibers， optimizing the SPME conditions， preparing environmental water samples， and analysis by HPLC. Guided by teachers， undergraduates learned relevant knowledge prior to performing the experiment， improved their operating skills during the experiment， analyzed data， and wrote research reports during the latter stage of the course. Undergraduates' interest is stimulated by completing the experiment， while scientific thinking， analysis， and problem-solving abilities are concurrently improved and cultivated. The present course also lays the foundation for cultivating future high-quality environmental-science talents.