Related Experiment Video

Updated: Jan 17, 2026

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
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In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

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N6-Methyladenosine-Modified circNEK11 Promotes Hepatocellular Carcinoma Progression via the miR-1236-3p/GPX2 Axis

Qian Li1,2,3,4, Xu Huang1,2,3,4, Yan Tong1,2,3,4

  • 1School of Pharmacy and Department of Hepatology, the Affiliated Hospital of Hangzhou Normal University, Hangzhou Normal University, Hangzhou, P. R. China.

Cancer Science
|September 24, 2025
PubMed

Insights

Circular RNA NEK11 (circNEK11) is upregulated in hepatocellular carcinoma (HCC), promoting tumor growth and recurrence. circNEK11 may serve as a diagnostic biomarker and therapeutic target for HCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) play regulatory roles in cancers.
  • The specific function of circNEK11 in hepatocellular carcinoma (HCC) is not well understood.

Purpose of the Study:

  • Investigate the role and mechanism of circNEK11 in HCC progression.
  • Determine circNEK11's potential as a biomarker for HCC.

Main Methods:

  • Quantified circNEK11 expression in HCC tissues.
  • Studied circNEK11's interaction with YTHDC1 and miR-1236-3p.
  • Assessed circNEK11's impact on HCC cell functions in vitro and in vivo.

Main Results:

  • circNEK11 expression is significantly upregulated in HCC, particularly in recurrent cases.
  • circNEK11 promotes HCC cell proliferation, migration, and tumor growth.
  • circNEK11 directly targets miR-1236-3p, regulating GPX2 expression and promoting HCC progression.

Conclusions:

  • circNEK11 acts as an oncogenic circRNA in HCC by regulating the miR-1236-3p/GPX2 axis.
  • circNEK11 is a potential diagnostic biomarker and therapeutic target for HCC.
Keywords:
GPX2circNEK11hepatocellular carcinomamiR‐1236‐3pprogression

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