DACN-Based Switchable Fluorescent Click Reaction
Yuuya Kawasaki1,2, Tomoya Hayashibara2, Kazunobu Igawa1,2
1Institute for Materials Chemistry and Engineering and IRCCS, Kyushu University, Kasuga-koen 6-1, Kasuga, Fukuoka 816-8580, Japan.
The Journal of Organic Chemistry
|September 25, 2025
Abstract:
The copper-free switchable fluorescent click reaction using 4,8-diazacyclononyne (DACN) and 3-azido-7-alkoxycoumarin was realized and applied to the conjugation of biomolecules. This new method using DACN has characteristics superior to those of the corresponding azadibenzocyclooctyne (DBCO) derivative in terms of fluorescence quantum yield and photostability under UV light irradiation.
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