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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.7K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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In-vitro Mutagenesis01:16

In-vitro Mutagenesis

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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Related Experiment Video

Updated: Jan 17, 2026

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera H&#252;bner
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Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

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CRISPR/Cas9-Mediated Gene Knockout in Cereal Crops.

Dibyajyoti Pramanik1,2, Kan Wang1,2, Keunsub Lee1,2

  • 1Department of Agronomy, Iowa State University, Ames, Iowa.

Current Protocols
|September 25, 2025
PubMed
Summary

The CRISPR/Cas9 system enables precise genome editing in plants, facilitating gene knockout for crop improvement. This guide details essential strategies for implementing CRISPR/Cas9 in plant research and breeding.

Keywords:
CRISPRcereal cropsgenome editingindelsmaizericesorghum

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Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
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Area of Science:

  • Plant Science
  • Genetics
  • Biotechnology

Background:

  • CRISPR/Cas9 is a powerful genome editing tool utilizing RNA-guided nucleases.
  • It introduces targeted DNA breaks, often leading to gene knockout via indel mutations.
  • This technology has been widely adopted in major crops like rice, maize, wheat, and sorghum.

Purpose of the Study:

  • To outline strategies for implementing CRISPR/Cas9 genome editing in plants.
  • To provide a step-by-step guide for researchers and breeders.
  • To highlight the application of CRISPR/Cas9 in improving crops for food security and other uses.

Main Methods:

  • Guide RNA target selection and oligonucleotide design.
  • CRISPR/Cas9 construct development and assembly.
  • Analysis of genome edits, including genotyping and plant transformation.

Main Results:

  • Successful application of CRISPR/Cas9 in various cereal crops.
  • Generation of targeted gene knockouts for trait improvement.
  • Established protocols for efficient genome editing in plants.

Conclusions:

  • CRISPR/Cas9 is a transformative technology for plant breeding and research.
  • The outlined strategies facilitate the effective implementation of genome editing in diverse crop species.
  • This approach supports advancements in global food security, nutrition, and sustainable agriculture.