Dual-function fluorescent sensor enabling real-time tracking of cellular viscosity and biothiols levels

Area of Science:

• Biochemistry
• Chemical Biology
• Biomedical Engineering

Background:

• Biothiols are crucial biomarkers for metabolic regulation and redox balance.
• Cellular microenvironment viscosity is linked to various human diseases.
• Fluorescent probes are valuable tools for detecting biomarkers.

Purpose of the Study:

• To construct a dual-function fluorescent probe (IDH-NBD) for simultaneous detection of biothiols and viscosity.
• To evaluate the probe's performance in vitro and in biological systems.
• To provide a new tool for disease prevention and early diagnosis.

Main Methods:

• Synthesis and characterization of the IDH-NBD fluorescent probe.
• In vitro studies to assess probe response to biothiols and viscosity.
• Biological imaging experiments in cells and zebrafish.

Main Results:

• The IDH-NBD probe demonstrated high sensitivity and efficiency for detecting biothiols and viscosity.
• The probe successfully monitored biothiols levels and viscosity in cellular and zebrafish models.
• Preliminary mechanistic studies of the probe's action were conducted.

Conclusions:

• The developed IDH-NBD probe is a versatile tool for real-time monitoring of biothiols and viscosity.
• This probe offers potential for advancing the early diagnosis and prevention of related diseases.
• The study highlights the utility of dual-function fluorescent probes in biomedical research.