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LRRC15 Promotes the Development of Breast Cancer by Regulating Autophagy through the PI3K/AKT/mTOR Signaling Pathway.
Xinru Fan1, Liu Gao2, Zheng Zhang1
1Key Laboratory of Cancer Research and Clinical Laboratory Diagnosis, School of Laboratory Medicine, Bengbu Medical University, Bengbu, China.
Cancer Research and Treatment
|September 25, 2025
Summary
Leucine-rich repeat-containing protein 15 (LRRC15) promotes breast cancer progression by inhibiting autophagy and activating the PI3K/AKT/mTOR pathway, leading to a worse prognosis.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Leucine-rich repeats (LRR) are implicated in tumorigenesis and cancer therapy.
- Leucine-rich repeat-containing protein 15 (LRRC15) expression is elevated in various cancers.
- The role of LRRC15 in breast cancer and its association with autophagy remain unclear.
Purpose of the Study:
- To investigate the expression and prognostic significance of LRRC15 in breast cancer.
- To elucidate the functional role of LRRC15 in breast cancer cell proliferation, migration, and invasion.
- To explore the mechanistic link between LRRC15, autophagy, and the PI3K/AKT/mTOR signaling pathway in breast cancer.
Main Methods:
- Bioinformatic analysis of LRRC15 expression and prognosis.
- Immunohistochemistry and qRT-PCR for LRRC15 expression in clinical samples.
- Cellular assays (clonogenic, MTT, wound healing, Transwell) for proliferation, migration, and invasion.
- Western blotting to assess autophagy and PI3K/AKT/mTOR pathway proteins.
Main Results:
- Elevated LRRC15 expression correlates with a worse prognosis and increased tumor aggressiveness in breast cancer patients.
- LRRC15 enhances breast cancer cell proliferation, migration, and invasion.
- LRRC15 inhibits autophagy in breast cancer cells, modulating their proliferative capacity.
- LRRC15 activates the PI3K/AKT/mTOR signaling pathway.
Conclusions:
- LRRC15 is a potential biomarker for breast cancer prognosis.
- LRRC15 promotes breast cancer progression by inhibiting autophagy via the PI3K/AKT/mTOR pathway.
- Targeting LRRC15 or the PI3K/AKT/mTOR pathway may offer therapeutic strategies for breast cancer.
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