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Morphometric Analyses of Retinal Sections
Published on: February 19, 2012
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Targeting Bcl3/NF-κB p50 Pathway for Neuroinflammation Attenuation and RGCs Protection in Retinal
Meini Chen1,2,3,4,5, Xuan Zhang1,2,3,4, Zhou Zeng1,2,3,4
1Eye Center of Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
Investigative Ophthalmology & Visual Science
|September 26, 2025
Summary
Resveratrol (RSV) protects retinal ganglion cells (RGCs) from injury by reducing inflammation and cell death. This study identifies the Bcl3/NF-κB p50 pathway as a key mechanism for RSV
Area of Science:
- Ophthalmology
- Neuroscience
- Pharmacology
Background:
- Glaucoma involves retinal ganglion cell (RGC) death due to ischemia/reperfusion (IR) injury and inflammation, often linked to high intraocular pressure (IOP).
- Current treatments lowering IOP are insufficient, necessitating neuroprotective strategies to prevent RGC loss.
- Resveratrol (RSV) shows potential anti-inflammatory and neuroprotective effects, but its precise molecular targets in retinal IR injury are not fully understood.
Purpose of the Study:
- To investigate the neuroprotective role of Resveratrol (RSV) in a model of retinal ischemia/reperfusion (IR) injury.
- To elucidate the underlying molecular mechanisms by which RSV exerts its protective effects against RGC death and inflammation.
Main Methods:
- Assessed retinal morphology, RGC survival, and function using immunofluorescence, H&E staining, flash visual evoked potential (F-VEP), and flash electroretinogram (F-ERG).
- Analyzed inflammation and microglial activation via qRT-PCR and immunohistochemistry.
- Examined pyroptosis and apoptosis using Western blotting, TUNEL staining, and electron microscopy; identified molecular pathways via RNA sequencing and qRT-PCR.
Main Results:
- RSV significantly preserved RGCs and retinal function, while reducing inflammation and microglial activation.
- Electron microscopy confirmed RSV's protective effects against apoptosis and pyroptosis.
- The Bcl3/NF-κB p50 pathway was identified as a key target of RSV, with inhibition of this pathway validating its role in reducing neuroinflammation and cell death.
Conclusions:
- Resveratrol demonstrates significant neuroprotective effects in retinal IR injury by modulating inflammation and cell death pathways.
- The Bcl3/NF-κB p50 pathway is a critical molecular target for RSV's therapeutic action.
- This research provides novel insights into RSV's mechanisms and identifies potential therapeutic targets for glaucoma treatment.
