Multispectral Pulsed Photobiomodulation Enhances Re-Epithelialization via Keratinocyte Activation in Full-Thickness...

Area of Science:

• Biomedical Engineering
• Dermatology
• Regenerative Medicine

Background:

• Chronic wound healing is a complex biological process involving epithelial and stromal regeneration.
• Photobiomodulation (PBM) with low-level light therapy is a promising non-invasive method for enhancing skin repair.
• Pulsed, multi-wavelength LED systems offer a tunable approach to PBM.

Purpose of the Study:

• To evaluate the therapeutic efficacy of a pulsed, multi-wavelength LED system on full-thickness excisional wound healing in a murine model.
• To investigate the effects of PBM on key cellular and molecular markers of skin regeneration.
• To assess the potential of PBM as a treatment for accelerating cutaneous wound healing.

Main Methods:

• Utilized a normal murine model with full-thickness excisional wounds.
• Administered daily treatment with a pulsed, multi-wavelength LED system.
• Conducted histological, immunohistochemical, and molecular profiling analyses.
• Performed in vitro assays using HaCaT keratinocytes.

Main Results:

• LED treatment significantly accelerated wound closure and re-epithelialization.
• Improved epidermal stratification, reduced inflammation, and enhanced collagen organization were observed.
• Increased expression of proliferation markers (Ki67) and matrix-related genes (MMPs, Col1a1, Col3a1) was noted.
• In vitro studies showed accelerated keratinocyte scratch wound closure and cytoskeletal remodeling.

Conclusions:

• Pulsed PBM effectively promotes coordinated epithelial regeneration and dermal matrix remodeling.
• The multi-wavelength LED system demonstrates significant therapeutic potential for accelerating cutaneous wound healing.
• PBM represents a tunable and effective modality for improving skin repair under physiological conditions.