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Whole-genome sequencing and assembly of fish pathogenic Proteus mirabilis (COFI-RLBCAU-II) possessing multiple

Partha Sarathi Tripathy1, Satya Narayan Parida1, Anuj Tyagi1

  • 1College of Fisheries (Datia), Rani Lakshmi Bai Central Agricultural University, Jhansi, Uttar Pradesh, India.

Microbiology Resource Announcements
|September 26, 2025
PubMed

Insights

A multidrug-resistant bacterium, Proteus mirabilis, was identified in diseased Labeo catla. This fish pathogen has a genome of 3.93 Mb containing 3,656 genes.

Area of Science:

  • Aquatic microbiology
  • Fish pathology
  • Genomics

Background:

  • Fish diseases pose significant threats to aquaculture and wild populations.
  • Antimicrobial resistance in fish pathogens complicates treatment and increases economic losses.
  • Proteus mirabilis is a known opportunistic pathogen in various hosts.

Purpose of the Study:

  • To identify and characterize a fish pathogenic bacterium isolated from diseased Labeo catla.
  • To investigate the antimicrobial resistance profile of the isolated bacterium.
  • To perform whole-genome sequencing of the identified pathogen.

Main Methods:

  • Isolation and identification of the bacterial strain from diseased fish.
  • Antimicrobial susceptibility testing.
  • Whole-genome sequencing and bioinformatic analysis.

Main Results:

  • A multidrug-resistant strain of Proteus mirabilis (COFI-RLBCAU-II, CP181371) was isolated from Labeo catla.
  • The bacterium belongs to the Enterobacteriaceae family.
  • The complete genome sequence was determined to be 3.93 Mb with 3,656 predicted genes.

Conclusions:

  • Proteus mirabilis is a significant fish pathogen contributing to disease outbreaks in Labeo catla.
  • The multidrug resistance of this isolate necessitates careful management strategies in aquaculture.
  • The genomic data provides a foundation for understanding virulence and resistance mechanisms.
Keywords:
fish pathogens

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