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Optimization of Manuka Honey microbubble particle structures for enhanced inhibition of Pseudomonas aeruginosa growth
1The Department of Commercial Design, National Taichung University of Science and Technology, Taichung 404336, Taiwan.
Abstract:
This study aims to optimize the use of Manuka Honey (MH) microbubbles for enhancing antimicrobial efficacy in the treatment of Pseudomonas aeruginosa (P. aeruginosa.) infections. The experimental design included three different MH microbubble particle sizes produced by three types of high-density stainless steel mesh layers, two types of dressing, two volumes of use, and three antimicrobial exposure times. The bacterial survival percentages of P. aeruginosa survival obtained under each combination of variables was calculated. The results demonstrated that MH microbubble size and antimicrobial exposure time were the key factors that improved growth inhibition. Additionally, the use of a high-density stainless steel mesh nozzle to produce MH microbubbles significantly enhanced inhibition of bacterial growth, with smaller microbubble sizes showing better inhibition (p < 0.05). Complete bacterial elimination (3.5 log) was achieved using either waterproof or foam dressings with three of nozzle layers (producing the microbubbles of 0.0016 mm) and 3 mL of microbubbles over 24 h.
Insights
Manuka Honey microbubbles effectively combat Pseudomonas aeruginosa infections. Optimized microbubble size and exposure time significantly enhance antimicrobial activity, leading to complete bacterial elimination.
Area of Science:
- Biomedical Engineering
- Microbiology
- Materials Science
Background:
- Pseudomonas aeruginosa infections pose a significant clinical challenge.
- Manuka Honey (MH) exhibits antimicrobial properties.
- Microbubble technology offers potential for enhanced drug delivery and efficacy.
Purpose of the Study:
- To optimize Manuka Honey (MH) microbubbles for improved antimicrobial efficacy against Pseudomonas aeruginosa (P. aeruginosa).
- To investigate the impact of microbubble particle size, dressings, volume, and exposure time on bacterial inhibition.
Main Methods:
- MH microbubbles were produced using high-density stainless steel mesh layers, varying particle sizes.
- Experimental design included different dressing types, volumes, and antimicrobial exposure times.
- Bacterial survival percentages of P. aeruginosa were quantified under various conditions.
Main Results:
- MH microbubble size and antimicrobial exposure time were critical factors for growth inhibition.
- High-density stainless steel mesh nozzles enhanced bacterial growth inhibition, with smaller microbubbles being more effective (p < 0.05).
- Complete elimination (3.5 log) of P. aeruginosa was achieved with specific parameters: 0.0016 mm microbubbles, 3 mL volume, 24h exposure, and waterproof or foam dressings.
Conclusions:
- Optimized Manuka Honey microbubbles demonstrate significant potential for treating P. aeruginosa infections.
- Microbubble size and production method are key determinants of antimicrobial efficacy.
- The study provides a foundation for developing advanced wound care strategies using MH microbubbles.
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