Polyacrylic acid/citrate/amorphous calcium phosphate complex for dentin remineralization and bond durability

Area of Science:

• Biomaterials Science
• Dental Materials Science
• Nanotechnology

Background:

• Dentin bionic remineralization is crucial for stable resin-dentin bonding.
• Conventional biomimetic methods have limitations in efficiency and applicability.
• Citrate plays a key role in biological mineralization processes.

Purpose of the Study:

• To prepare polyacrylic acid/citrate/amorphous calcium phosphate (PAA-Cit-ACP) complexes.
• To investigate the potential of PAA-Cit-ACP in promoting biomimetic mineralization.
• To evaluate the effect of PAA-Cit-ACP on the stability of the resin-dentin bonding interface.

Main Methods:

• Synthesis and characterization of four PAA-Cit-ACP complexes with varying citrate content.
• Molecular dynamics simulation to elucidate complex formation mechanisms.
• Mineralization assays using collagen fibers and dentin slices; nanoleakage and in situ zymography to assess bond durability.

Main Results:

• PAA-Cit-ACP nanoparticles exhibited good biocompatibility and promoted rapid mineralization.
• Treatment reduced nanoleakage and matrix metalloproteinase (MMP) activity at the resin-dentin interface.
• PAA-Cit-ACP-1 and PAA-Cit-ACP-2 formulations showed superior effectiveness.

Conclusions:

• PAA-Cit-ACP facilitates rapid biomimetic remineralization of dentin.
• It protects demineralized collagen from degradation, enhancing hybrid layer stability.
• This approach offers a promising strategy for improving resin-dentin bond longevity.