Comprehensive Evaluation of Toxicological Profile and Immunomodulatory Impact of an Immune Enhancing Oral Liquid

Traditional Chinese herbal medicines are characterized by multi-component synergy and have garnered significant attention in the healthcare research. The Immune Enhancing Oral Liquid, formulated with Polygonum multiflorum Thunb. and Morinda officinalis How extracts, has demonstrated biological activities including gut microbiota modulation. To systematically evaluate its safety profile, toxicity, and immune-enhancing efficacy, this study assessed toxicological and immunomodulatory parameters in murine models through the following protocols: acute toxicity testing; genotoxicity assays; 28-day oral toxicity study; and immunological function assessments encompassing cellular immunity, humoral immunity, non-specific immunity, and cytotoxic function. Results indicated that the immune enhancing oral liquid showed no obvious acute toxicity, genotoxicity, or 28-day oral toxicity. Furthermore, the immune enhancing oral liquid exhibited no adverse effects on murine hematopoietic or hepatic functions. Crucially, the immune enhancing oral liquid significantly enhanced immune responses, as evidenced by promoted lymphocyte proliferation, elevated serum hemolysin levels, augmented macrophage phagocytic activity, and heightened NK cell activity. Immune enhancing oral liquid was recognized as safe and non-toxic, and the study provides a scientific basis for the application of the oral liquid, validating the effectiveness of the combination of traditional herbal wisdom and modern pharmacology.