Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Botrytis elliptica</i> Infection Induces <i>LhSorPALs</i> Expression in <i>Lilium</i>: Overexpression of <i>LhSorPAL1</i> and <i>LhSorPAL2</i> Enhances Disease Resistance via Phenylpropane Metabolite Accumulation.

Plants (Basel, Switzerland)·2026
Same author

Fungal Metabolomics and Genomics: Integrating Multi-Omics to Decipher Function, Diversity, and Application.

Journal of fungi (Basel, Switzerland)·2026
Same author

A study on the relationship between mMRC dyspnea scale and both risk stratification and poor prognosis in patients with acute pulmonary embolism.

Frontiers in cardiovascular medicine·2026
Same author

Study on D6AC Steel PCBN Hard Turning and Optimization.

Materials (Basel, Switzerland)·2026
Same author

Biosynthesis-guided classification of Hirsutane sesquiterpenoids and their structural diversification.

Bioorganic chemistry·2026
Same author

Elucidating the Multi-Enzymatic Mechanism of Bacterial Decolorization of Azo and Indigoid Dyes: An Integrated Study of Degradation Pathways and Molecular Docking.

International journal of molecular sciences·2026
Same journal

The Potential for Bioactive Peptide Production in a Fermented Dairy Beverage Based on Chickpea Water Extract Using Proteolytic Lactic Acid Bacteria.

Foods (Basel, Switzerland)·2026
Same journal

Influence of Protein Concentration on Heat-Induced Fouling of Oat Drink.

Foods (Basel, Switzerland)·2026
Same journal

Microalgae as Future Foods: Unlocking Their Potential and Overcoming Barriers to Market Adoption and Commercialization.

Foods (Basel, Switzerland)·2026
Same journal

Effect of High-Intensity Ultrasound and Calcium Chelation on Functional Properties of Casein Micelles.

Foods (Basel, Switzerland)·2026
Same journal

GC-MS and GC-IMS Based Metabolomics Combined with Cellular Assays to Characterize Volatile Compounds and Pharmacological Activity of <i>Lysimachia foenum-graecum</i> Hance from Different Origins.

Foods (Basel, Switzerland)·2026
Same journal

Research on the Potential Mechanism of Guanine Nucleotides Enhancing the Tolerance of <i>Lactiplantibacillus plantarum</i> Y12.

Foods (Basel, Switzerland)·2026
See all related articles

Related Experiment Video

Updated: Jan 16, 2026

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
06:55

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

Published on: November 7, 2018

9.4K

Simultaneous Detection of Four Foodborne Pathogens in Raw Freshwater Fish Using High-Resolution Melting Analysis.

Shan Shan1,2, Xiaoyu Tong1, Wenyu Du1

  • 1Nanchang Key Laboratory of Microbial Resources Exploitation & Utilization from Poyang Lake Wetland, College of Life Science, Jiangxi Normal University, Nanchang 330022, China.

Foods (Basel, Switzerland)
|September 27, 2025
PubMed
Summary
This summary is machine-generated.

A new multiplex PCR-HRM assay simultaneously detects four key foodborne pathogens in raw fish, enhancing food safety. This method offers a simple, efficient, and cost-effective solution for pathogen detection in aquatic products.

Keywords:
food safetyfoodborne pathogenshigh-resolution melting (HRM)raw freshwater fish

More Related Videos

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis
06:30

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

Published on: February 5, 2014

22.9K
Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
06:12

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Published on: October 25, 2018

9.1K

Related Experiment Videos

Last Updated: Jan 16, 2026

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
06:55

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

Published on: November 7, 2018

9.4K
Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis
06:30

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

Published on: February 5, 2014

22.9K
Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
06:12

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Published on: October 25, 2018

9.1K

Area of Science:

  • Food Science
  • Microbiology
  • Molecular Biology

Background:

  • Raw fish consumption is popular globally but poses risks due to potential contamination with foodborne pathogens.
  • Key pathogens like Listeria monocytogenes, Salmonella, Vibrio parahaemolyticus, and Staphylococcus aureus are common threats in raw seafood.
  • Accurate and rapid detection methods are crucial for ensuring the safety of aquatic products.

Purpose of the Study:

  • To develop and validate a novel multiplex assay for the simultaneous detection of four major foodborne pathogens in raw fish.
  • To establish a high-resolution melting (HRM) curve analysis integrated with multiplex PCR for pathogen identification.
  • To assess the sensitivity, specificity, and efficiency of the developed assay for food safety applications.

Main Methods:

  • Multiplex polymerase chain reaction (PCR) was employed to amplify target genes from four specific foodborne pathogens.
  • High-resolution melting (HRM) curve analysis was utilized to differentiate the amplified products based on melting temperature (Tm).
  • The assay's performance was evaluated using various concentrations of genomic DNA, specificity testing with non-target strains, and sensitivity analysis.

Main Results:

  • The PCR-HRM assay successfully detected Listeria monocytogenes, Salmonella, Vibrio parahaemolyticus, and Staphylococcus aureus simultaneously.
  • The detection limit ranged from 0.02-0.1 ng/µL, demonstrating high sensitivity.
  • The assay exhibited excellent specificity and accuracy, with no interference from co-extracted DNA of non-target bacteria.

Conclusions:

  • The integrated multiplex PCR-HRM assay is a highly efficient, accurate, and cost-effective method for detecting multiple foodborne pathogens in raw fish.
  • This assay significantly reduces detection time and operational complexity, making it ideal for routine food safety monitoring.
  • The developed method provides a robust tool for enhancing safety standards in the aquatic products processing industry.