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EET-Based Therapeutics Mitigate Sorafenib-Associated Glomerular Cell Damage.

Abhishek Mishra1, Marcus de Bourg2, Rawand S Mohamed2

  • 1Department of Pharmaceutical Sciences, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.

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Summary

This study shows that 8,9-epoxyeicosatrienoic acid (8,9-EET) analogs protect kidney cells from sorafenib toxicity. One analog, MDB-52a, reduced cell death and counteracted gene changes linked to kidney damage.

Keywords:
epoxylipidsmesangial cellsnephrotoxicityonconephrologypodocytes

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Area of Science:

  • Nephrology
  • Molecular Biology
  • Pharmacology

Background:

  • Sorafenib, a tyrosine kinase inhibitor, can cause kidney damage.
  • Glomerular cells are key targets of sorafenib-induced nephrotoxicity.
  • 8,9-epoxyeicosatrienoic acid (8,9-EET) analogs are investigated for protective effects.

Purpose of the Study:

  • To investigate sorafenib-induced toxicity in human renal mesangial cells (HRMCs) and podocytes.
  • To examine the protective potential of 8,9-EET analogs against sorafenib-induced kidney damage.
  • To identify specific 8,9-EET analogs that mitigate sorafenib nephrotoxicity.

Main Methods:

  • HRMCs and podocytes were exposed to sorafenib alone or with 8,9-EET analogs.
  • Cell viability assays and caspase 3/7 activity measurements were performed.
  • RNA sequencing and analysis of public kidney gene expression data (Nephroseq) were utilized.

Main Results:

  • Sorafenib decreased cell viability and increased apoptosis in a dose-dependent manner.
  • Five of twenty 8,9-EET analogs demonstrated significant cytoprotective effects.
  • Sorafenib altered genes in cell cycle and Raf/MEK/ERK pathways; MDB-52a modulated ANGPTL4 and ACTA2 expression, correlating with glomerulosclerosis markers.

Conclusions:

  • The 8,9-EET analog MDB-52a shows promise in counteracting sorafenib-induced gene disruptions.
  • MDB-52a exhibits protective effects against sorafenib-related kidney damage in vitro.
  • Targeting glomerular cells with 8,9-EET analogs may offer a therapeutic strategy for sorafenib nephrotoxicity.