The Protective Mechanism of Moderate Intensity Continuous Training on TMAO-Induced Myocardial Injury Based on NMR...

Area of Science:

• Cardiovascular Research
• Metabolomics
• Exercise Physiology

Background:

• Trimethylamine N-oxide (TMAO) is linked to cardiovascular disease.
• Myocardial injury can result from TMAO exposure.
• Understanding metabolic mechanisms is crucial for treatment strategies.

Purpose of the Study:

• To investigate the protective effects of Moderate Intensity Continuous Training (MICT) against TMAO-induced myocardial injury in mice.
• To elucidate the metabolic regulatory mechanisms underlying MICT's protective effects using NMR metabolomics.

Main Methods:

• Male C57BL/6J mice were divided into Control, TMAO-induced injury, and TMAO + MICT groups.
• Eight weeks of high-dose TMAO gavage induced myocardial injury.
• MICT intervention involved 60 min/session, 5 sessions/week at 50% MRC.
• Cardiac function (ultrasound), histology (HE staining), and NMR metabolomics were assessed.

Main Results:

• TMAO increased heart rate (HR) and decreased ejection fraction (EF) and left ventricular fractional shortening (LVFS).
• MICT intervention reversed these changes, reducing HR and improving EF and LVFS.
• NMR metabolomics revealed significant alterations in phenylalanine, tyrosine, and TCA cycle metabolism in the TMAO group.
• MICT significantly regulated amino acid metabolism, energy metabolism (TCA cycle), and purine metabolism.

Conclusions:

• Moderate Intensity Continuous Training (MICT) effectively alleviates TMAO-induced myocardial injury in mice.
• MICT achieves this by regulating multiple myocardial metabolic pathways, including amino acid and energy metabolism.
• These findings support exercise intervention as a potential treatment for myocardial injury.