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09:01
Cancer-Associated Fibroblasts from Mouse Mammary Tumors as Tools for Molecular and Computational Studies
Published on: July 3, 2025
861
CYR61 Expression Is Induced by IGF1 and Promotes the Proliferation of Prostate Cancer Cells Through the PI3/AKT
Greisha L Ortiz-Hernández1, Carmina Patrick1, Stefan Hinz1
1Department of Population Sciences, Division of Biomarkers of Early Detection and Prevention, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.
International Journal of Molecular Sciences
|September 27, 2025
Summary
Cysteine-rich angiogenic inducer 61 (CYR61) drives prostate cancer (PCa) progression by interacting with insulin-like growth factor-1 (IGF1). Inhibiting CYR61 reduces PCa cell growth and metastasis, suggesting it as a therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Cysteine-rich angiogenic inducer 61 (CYR61) is implicated in prostate cancer (PCa) cell growth.
- The precise role of CYR61 in PCa progression and its interaction with insulin-like growth factor-1 (IGF1) are not fully understood.
Purpose of the Study:
- To investigate the molecular interplay between CYR61 and IGF1 in prostate cancer.
- To determine the effect of CYR61 on PCa cell proliferation, viability, migration, and signaling pathways.
Main Methods:
- CYR61 was silenced using small interfering RNA (siRNA) in PC3, LNCaP, and 22Rv1 prostate cancer cell lines.
- Cell proliferation, viability, colony formation, migration, and signaling pathway activation (PI3K/AKT, MAPK) were assessed.
- The effect of recombinant IGF1 on CYR61 expression and cell proliferation was evaluated.
Main Results:
- CYR61 knockdown significantly reduced proliferation, viability, prostasphere formation, and migration in all tested PCa cell lines.
- CYR61 silencing inhibited the PI3K/AKT signaling pathway but did not affect MAPK activation.
- Recombinant IGF1 treatment increased CYR61 expression and cell proliferation, which was attenuated by PI3K/AKT inhibition.
Conclusions:
- Insulin-like growth factor-1 (IGF1) promotes prostate cancer progression via Cysteine-rich angiogenic inducer 61 (CYR61).
- CYR61 plays a crucial role in PCa cell growth, viability, and metastasis.
- CYR61 represents a potential therapeutic target for inhibiting prostate cancer progression and metastasis.
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