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Updated: Jul 30, 2026

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry
Published on: October 2, 2016
Rapid Identification of Dendrobium Species Using Near-Infrared Hyperspectral Imaging Technology
Kaixuan Li1, Yijun Guo1, Haosheng Zhong2
1College of Optical, Mechanical and Electrical Engineering, Zhejiang A&F University, Hangzhou 311300, China.
Abstract:
Dendrobium officinale is a valuable Chinese medicinal herb, but distinguishing it from other Dendrobium species after processing is challenging, leading to low classification accuracy and time-consuming analysis. This study proposes a rapid classification model based on near-infrared hyperspectral imaging (NIR-HSI), incorporating data preprocessing and feature wavelength selection. Five Dendrobium species-D. officinale, D. aphyllum, D. chrysanthum, D. fimbriatum, and D. thyrsiflorum-were used. Spectral preprocessing techniques like normalization and smoothing were applied, and Support Vector Machine (SVM) models were constructed. Normalization improved both accuracy and stability, with the full-spectrum Normalize-SVM model achieving 97% accuracy for calibration and 88% for prediction. D. chrysotoxum performed best, with all metrics reaching 100%, while D. aphyllum had poor classification (40% recall and 51.74% F1 score). To improve efficiency and performance, feature wavelength selection was performed using Competitive Adaptive Reweighted Sampling (CARS) and Successive Projections Algorithm (SPA). The CARS-Normalize-SVM model yielded the best results: 98% accuracy for calibration and 96% for prediction, improving by 1% and 8%, respectively. D. aphyllum's classification also improved significantly, with a 100% recall rate and 95.24% F1 score. These findings highlight hyperspectral imaging's potential for rapid Dendrobium species identification, supporting future quality control and market supervision.
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