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Prochirality

Prochirality

The concept of prochirality leads to the nomenclature of the individual faces of a molecule and plays a crucial role in the enantioselective reaction. It is a concept where two or more achiral molecules react to produce chiral products. A typical process is the reaction of an achiral ketone to generate a chiral alcohol. Here, the achiral reactant reacts with an achiral reducing agent, sodium borohydride, to generate an equimolar mixture of the chiral enantiomers of the product. For example, an...

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Related Experiment Video

Updated: Jan 16, 2026

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

A Rational Design Strategy for Engineering CH4 Domain of IgE for Heterodimerization.

Shikha Kumari^1,2,3, Vanessa Siegmund⁴, Achim Doerner⁴

¹Manipal Academy of Higher Education, Manipal, Karnataka, India.

Biotechnology and Bioengineering

|September 27, 2025

Summary

This summary is machine-generated.

Researchers developed a computational method to create bispecific immunoglobulin E (IgE) antibodies for cancer immunotherapy. This novel approach enables the design of IgE bispecifics, enhancing potential treatments for solid tumors.

Keywords:

antibody engineering bispecific IgE recombinant IgE therapeutic IgE

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Identifying Protein-protein Interaction Sites Using Peptide Arrays

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Published on: November 18, 2014

Area of Science:

Immunology
Biotechnology
Oncology

Background:

Bispecific antibodies are increasingly vital in cancer immunotherapy, with nine approvals between 2021-2023.
Exploration of immunoglobulin E (IgE) isotypes shows potential for superior anti-tumor activity compared to immunoglobulin G (IgG) in certain cancers.
Current limitations in developing bispecific IgE antibodies stem from a lack of effective heterodimerization technologies.

Purpose of the Study:

To computationally design heterodimeric IgE antibodies.
To adapt the established IgG knob-into-hole (KiH) strategy for IgE antibody engineering.
To establish a proof of concept for developing novel therapeutic bispecific IgE antibodies.

Main Methods:

Utilized a computational approach to design heterodimeric IgE antibodies.
Applied the principles of the IgG knob-into-hole (KiH) strategy to IgE.
Validated the functionality of the designed bispecific IgE variant.

Main Results:

Successfully designed a novel variant of heterodimeric IgE antibodies.
The engineered IgE bispecific retained essential target binding capabilities.
Fc epsilon receptor engagement was confirmed for the novel IgE variant.

Conclusions:

A computational strategy for designing heterodimeric IgE antibodies was successfully developed.
This work provides a proof of concept for the future development of therapeutic bispecific IgE antibodies.
The engineered bispecific IgE shows promise for enhancing cancer immunotherapy, particularly for solid tumors.