Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Buckling-Resistant and Trace-Stacked (BRATS) Design Enables Aid-Free Implantation of Flexible Multielectrode Array with Minimized Inflammatory Tissue Response.

Advanced functional materials·2026
Same author

Effective General Practice-Led Interventions to Increase Uptake in Colorectal Cancer Screening: A Scoping Review.

Journal of public health management and practice : JPHMP·2026
Same author

Chronic alteration of Ca<sup>2+</sup> and hemodynamic signals induced by intracortical microstimulation in the visual cortex of awake mice.

Biomaterials·2026
Same author

Online Charge Balancing With Active Impedance Monitoring in Programmable Stimulator to Extend Electrode Lifetime.

IEEE access : practical innovations, open solutions·2026
Same author

Solving the problem of inception: a cross-species perspective on strategies for a mechanistic refinement of intracortical microstimulation.

Journal of neural engineering·2026
Same author

In vivo microelectrode arrays for neuroscience.

Nature reviews. Methods primers·2026
Same journal

In-situ cascade assembled peptide-drug conjugate for the treatment of bladder cancer by enhancing membrane-entry and lysosome destabilization.

Biomaterials·2026
Same journal

Antifibrotic monocyte activation by nanoparticles resolves murine pulmonary fibrosis.

Biomaterials·2026
Same journal

Versatile hollow Ca<sup>2+</sup>-phenolic nanoparticles for intracellular delivery of diverse bioactive molecules and CRISPR-Cas9 genome editing.

Biomaterials·2026
Same journal

Sequential targeting nanochaperone disrupts positive feedback loop of mitochondrial dysfunction for Alzheimer's disease therapy.

Biomaterials·2026
Same journal

Charge transfer modulation in anionic Cy5-Protein afterglow nanoprobe enables precise pancreatic cancer surgery and efficient metastasis inhibition.

Biomaterials·2026
Same journal

Size switchable nanomodulator achieving ratio-precise dual-drug codelivery for synergistic glutamine metabolism modulation in pancreatic cancer.

Biomaterials·2026
See all related articles

Related Experiment Video

Updated: Jan 16, 2026

Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices
07:27

Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices

Published on: January 31, 2019

10.3K

Intracortical microstimulation induces rapid microglia process convergence.

Nathaniel P Williams1, Anna M Kelly2, X Sally Zheng2

  • 1Department of Bioengineering, University of Pittsburgh, United States; Center for Neural Basis of Cognition, University of Pittsburgh and Carnegie Mellon University, United States.

Biomaterials
|September 28, 2025
PubMed
Summary
This summary is machine-generated.

Intracortical microstimulation (ICMS) rapidly activates microglia and compromises blood-brain barrier integrity in mice. These findings highlight the need for further research into ICMS biological responses and safety standards.

More Related Videos

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
08:26

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

Published on: August 23, 2022

2.9K
Multiscale Investigations of Cortical Processing by Integrating Laminar Polytrodes and Optogenetics with Micro Electrocorticography in Rodents
07:52

Multiscale Investigations of Cortical Processing by Integrating Laminar Polytrodes and Optogenetics with Micro Electrocorticography in Rodents

Published on: May 23, 2025

787

Related Experiment Videos

Last Updated: Jan 16, 2026

Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices
07:27

Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices

Published on: January 31, 2019

10.3K
Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
08:26

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

Published on: August 23, 2022

2.9K
Multiscale Investigations of Cortical Processing by Integrating Laminar Polytrodes and Optogenetics with Micro Electrocorticography in Rodents
07:52

Multiscale Investigations of Cortical Processing by Integrating Laminar Polytrodes and Optogenetics with Micro Electrocorticography in Rodents

Published on: May 23, 2025

787

Area of Science:

  • Neuroscience
  • Biomedical Engineering

Background:

  • Intracortical microstimulation (ICMS) shows promise for sensory restoration.
  • Neuronal responses to ICMS are well-studied, but non-neuronal cell responses and blood-brain barrier (BBB) effects are unclear.

Purpose of the Study:

  • To investigate microglial responses and BBB integrity during ICMS.
  • To characterize the biological effects of clinically relevant ICMS parameters.

Main Methods:

  • Used dual-reporter mice for simultaneous imaging of microglia and neurons.
  • Applied ICMS with clinically relevant waveforms and varying current amplitudes.
  • Monitored microglial process morphology and vascular dye leakage to assess BBB integrity.

Main Results:

  • Microglia rapidly converged their processes (MPC) on active neural areas within 15 minutes of ICMS.
  • The prevalence of MPC increased with higher current amplitudes.
  • Increased vascular dye penetration indicated compromised BBB integrity, also dose-dependent on current amplitude.

Conclusions:

  • ICMS elicits rapid, previously unreported responses in microglia and affects BBB integrity.
  • Findings suggest a need for further research to understand ICMS biological impacts.
  • This research emphasizes the importance of establishing robust safety standards for ICMS applications.