Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

1.4K
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
1.4K
Mass Spectrometers01:16

Mass Spectrometers

8.3K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
8.3K
Chemical Ionization (CI) Mass Spectrometry01:21

Chemical Ionization (CI) Mass Spectrometry

1.4K
The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
1.4K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.3K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.3K
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

2.7K
An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
2.7K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

1.0K
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
1.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hybrid dissociation modes in a portable ion trap mass spectrometer for enhanced specificity in complex mixture analysis.

Analytica chimica acta·2026
Same author

Risk factors and nomogram prediction model for isolated distal deep vein thrombosis after endovascular treatment in acute ischemic stroke patients.

Frontiers in neurology·2026
Same author

Towards Unified Semantic and Controllable Image Fusion: A Diffusion Transformer Approach.

IEEE transactions on pattern analysis and machine intelligence·2025
Same author

Non-loss engraved circuit patterning method of semi-liquid metal for precision recyclable multi-substrate circuits.

Nature communications·2025
Same author

SMURF2-Mediated Ubiquitination and Degradation of HOXA5 Augments Endoplasmic Reticulum Stress in Alveolar Epithelial Type II Cells Induced by LPS.

Molecular biotechnology·2025
Same author

Kynurenine pathway: a possible new mechanism for exercise in the prevention and treatment of Alzheimer's disease.

Frontiers in aging neuroscience·2025

Related Experiment Video

Updated: Jan 16, 2026

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
08:43

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

12.9K

Portable proton transfer reaction ion trap mass spectrometer with selectable buffer gas.

Jun Wang1, La Chen1,2, Chengjie Jiang1

  • 1The Research Institute of Advanced Technologies, Ningbo University, Ningbo, 315211, China. chenla@nbu.edu.cn.

The Analyst
|September 29, 2025
PubMed
Summary

A new miniaturized Proton Transfer Reaction Mass Spectrometry (PTR-MS) device offers rapid, on-site volatile organic compound analysis. This portable instrument achieves high sensitivity and improved performance using helium buffer gas.

More Related Videos

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
09:18

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Published on: April 17, 2017

10.3K
Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
09:34

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Published on: September 20, 2016

11.6K

Related Experiment Videos

Last Updated: Jan 16, 2026

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
08:43

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

12.9K
Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
09:18

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Published on: April 17, 2017

10.3K
Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
09:34

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Published on: September 20, 2016

11.6K

Area of Science:

  • Analytical Chemistry
  • Instrument Development

Background:

  • Proton Transfer Reaction Mass Spectrometry (PTR-MS) is crucial for detecting volatile organic compounds (VOCs).
  • Conventional PTR-MS devices are bulky, limiting real-time, on-site applications.
  • There is a need for portable, sensitive instruments for field analysis.

Purpose of the Study:

  • To develop a miniaturized PTR-MS instrument for on-site VOC analysis.
  • To evaluate the performance of the miniaturized PTR-MS using different buffer gases.
  • To assess the instrument's sensitivity, resolution, and detection limits.

Main Methods:

  • Development of a portable PTR-MS based on a portable ion trap mass spectrometer.
  • Utilized a small hollow-cathode discharge for ion generation and a compact ion funnel drift tube.
  • Investigated performance using helium and nitrogen as buffer gases, analyzing mass resolution, ion fragmentation, and sensitivity.

Main Results:

  • The miniaturized PTR-MS weighs 25 kg.
  • Helium buffer gas doubled mass resolution and increased ion fragmentation by 17x compared to nitrogen.
  • Achieved limits of detection of 1 ppbv for acetone, 0.5 ppbv for benzene, and 0.1 ppbv for toluene.
  • Established linear calibration curves over two orders of magnitude concentration range.

Conclusions:

  • The developed miniaturized PTR-MS is suitable for rapid, on-site VOC analysis.
  • Helium buffer gas significantly enhances instrument performance.
  • The portable instrument demonstrates high sensitivity and potential for environmental and industrial applications.