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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Related Experiment Video

Updated: Jan 16, 2026

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

Single-Cell Proteomic Technologies: Tools in the quest for principles.

|September 29, 2025

Summary

This summary is machine-generated.

Proteomic analysis of single cells using mass spectrometry has advanced significantly, enabling accurate protein quantification. Future developments promise increased throughput and functional insights for biophysical modeling.

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Published on: November 15, 2017

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Published on: April 21, 2022

Related Experiment Videos

Last Updated: Jan 16, 2026

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Published on: April 21, 2022

Area of Science:

Biochemistry
Proteomics
Cell Biology

Background:

Mass spectrometry-based proteomic analysis of single cells has evolved rapidly over the past decade.
Initial feasibility studies have given way to robust and scalable technologies.

Purpose of the Study:

To review the key advancements in single-cell proteomic analysis by mass spectrometry.
To analyze the tradeoffs and synergies of various technological solutions.
To project future directions for throughput scaling and functional protein measurements.

Main Methods:

Review of technological progress in single-cell proteomics.
Analysis of different mass spectrometry-based approaches.
Conceptual framework for evaluating scalability and scope.

Main Results:

Significant progress has been made, moving from uncertain possibility to reliable methods.
Accurate quantification of thousands of proteins is now achievable.
Considerable potential exists for scaling throughput and expanding to functional measurements.

Conclusions:

Single-cell proteomics by mass spectrometry is a powerful and advancing field.
These technologies offer potential for developing mechanistic biophysical models.
Further innovation can uncover new biological principles.