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Updated: Jan 25, 2026

Principles of Site-Specific Recombinase SSR Technology

Principles of Site-Specific Recombinase SSR Technology

Published on: May 29, 2008

Single-Cell Proteomic Technologies: Tools in the Quest for Principles.

Nikolai Slavov^1,2

¹Department of Bioengineering, Department of Biology, Department of Chemistry and Chemical Biology, Single Cell Proteomics Center, and Barnett Institute for Chemical and Biological Analysis, Northeastern University, Boston, Massachusetts, USA;

Annual Review of Biophysics

|January 23, 2026

Summary

This summary is machine-generated.

Proteomics analysis of single cells using mass spectrometry has advanced significantly, enabling accurate quantification of thousands of proteins. Future developments promise increased throughput and functional protein measurements for biophysical modeling.

Keywords:

biophysical models causal inference mass spectrometry sample preparation single-cell proteomics statistical inference

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Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

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Published on: August 19, 2025

Area of Science:

Biochemistry
Analytical Chemistry
Cell Biology

Background:

Single-cell proteomics analysis via mass spectrometry has evolved from a nascent concept to a robust technology.
Accurate quantification of thousands of proteins in single cells is now achievable.

Purpose of the Study:

To review the progress and key drivers in single-cell proteomics.
To analyze technological trade-offs and synergies for scalability and expanded analytical scope.
To highlight the potential for developing mechanistic biophysical models and uncovering new biological principles.

Main Methods:

Review of advancements in mass spectrometry-based single-cell proteomics.
Analysis of technological solutions for protein quantification and scalability.
Exploration of methods for functional protein measurements.

Main Results:

Significant progress has been made in establishing the feasibility and robustness of single-cell proteomics.
Current technologies offer accurate quantification of thousands of proteins.
There is substantial potential for scaling throughput and extending analysis to functional protein measurements.

Conclusions:

Single-cell proteomics is a rapidly advancing field with considerable potential for future development.
These technologies can support the creation of mechanistic biophysical models.
Further advancements may lead to the discovery of novel biological principles.