Rapid detection of Senecavirus A using a colloidal gold lateral flow strip

Senecavirus A (SVA) could cause vesicular lesions and other symptoms in pigs, which has resulted in substantial economic losses to the swine industry. To establish a rapid detection method for SVA, an immunochromatographic lateral flow strip was constructed. First, the recombinant SVA VP2 protein was purified, and the monoclonal antibodies were prepared. 4B10 was coated onto the test line (T line), and 1E3 was colloidal gold-labeled to assemble the colloidal gold test strip. The constructed LFA strip showed a detection limit of 0.5 ng/mL, and no cross-reactivity with FMDV, PRRSV, CSFV and PCV2. Clinical samples validation showed 90 % concordance with RT-PCR results, indicated the reliability of this method. The developed lateral flow assay (LFA) provided a rapid and simple method for point-of-care testing of SVA.