Development of an Innovative LED-based Illumination Device for In Vitro Application of Photodynamic Therapy with Rose...

Area of Science:

• Biomedical Engineering
• Photodynamic Therapy
• Cancer Research

Background:

• Photodynamic therapy (PDT) is a promising cancer treatment modality.
• Rose Bengal (RB) is a photosensitizer used in PDT, with peak absorption around 550 nm.
• Standardized in vitro PDT evaluation requires specialized equipment.

Purpose of the Study:

• To introduce and validate a novel 3D-printed device, CELL-LED-550/3, for in vitro RB-mediated PDT.
• To assess the device's capability in controlling light delivery for PDT experiments.
• To demonstrate the efficacy of RB-mediated PDT using the device on hepatocellular carcinoma cells.

Main Methods:

• Development of a 3D-printed device (CELL-LED-550/3) with a 550 nm LED light source and a 96-well plate light distributor.
• Three irradiance levels (0.02, 0.23, 0.62 mW/cm²) were delivered to HepG2 cells treated with varying RB concentrations.
• Cell viability was assessed 24 hours post-PDT using Cell-Titer Glo assay after illumination at different light doses (0.3, 0.6, 1.2 J/cm²).

Main Results:

• The CELL-LED-550/3 device successfully delivered controlled light doses for PDT.
• RB-mediated PDT using the device significantly decreased HepG2 cell viability.
• The observed cell death was dependent on both RB concentration and light dose administered.

Conclusions:

• The CELL-LED-550/3 device is a functional tool for in vitro studies of RB-mediated PDT.
• This device facilitates standardized and reproducible PDT experiments.
• The study confirms the potential of RB-mediated PDT for targeting hepatocellular carcinoma cells in vitro.