High-performance antifouling electrochemical aptsensor based on a Y-shaped glycopeptide and platinum nanoparticles...

Challenge-associated fouling aroused by non-specific attachment of non-target substances on sensor surface from complex food matrix urgently needs to be addressed. Herein, a Y-shaped glycopeptide-based electrochemical aptasensor with highly efficient antifouling capacity was proposed. The glycopeptide (CPPPPEK[KS(Glc)RE]DER) was designed by integrating a glucose molecule onto one of the side chain of the Y-shaped peptide and outperformed in antifouling performance in both simulated and real food matrices compared to not only the EK-based frequently reported linear peptide but also the original Y-shaped peptide. Molecular dynamics (MD) simulations further explained its antifouling ability from the molecular hydration behavior. Platinum nanoparticles (Pt NPs) were electrodeposited onto the electrode surface to enhance conductivity and immobilize glycopeptides and aptamers to construct the antifouling electrochemical aptasensor. The proposed strategy supports accurate analysis of aflatoxin B1 (AFB1) in soy sauce, milk powder, chestnuts with satisfactory recoveries within 100.3 %-111.5 %, showing its great potential for practical applications.