Association of SIRT1 rs12415800 and SIRT6 rs350844 polymorphisms with preeclampsia: A case-control study

SIRT1 and SIRT6, key members of the Sirtuins (SIRTs) family, play crucial roles in regulating cell metabolism and survival, providing beneficial effects against aging and metabolic diseases, which may be involved in the pathogenesis of preeclampsia (PE) by modulating cell senescence phenotypes in the placenta through antioxidant and inflammatory responses. This study aimed to investigate the association of SIRT1 and SIRT6 polymorphisms with PE in Chinese Han women.

A total of 619 healthy pregnant women were enrolled as controls, and 699 pregnant women with PE as cases. DNA was extracted from peripheral blood samples and was used for genotyping rs12415800 in SIRT1 and rs350844 in SIRT6 via real-time quantitative polymerase chain reaction (PCR). The relationship between SIRT1 and SIRT6 polymorphisms and PE was assessed by comparing genotypic and allelic frequencies between the two groups.

The results indicated that the SIRT1 rs12415800 polymorphism may be associated with PE risk and the AA genotypes was associated with a lower risk of developing PE compared to the AG and GG (genotype χ2 = 6.612, P = 0.037; allele χ2 = 1.279, P = 0.258; OR = 0.720, 95 % CI = 0.548 ∼ 0.951, P = 0.002). Subgroup analyses (early-onset vs. late-onset PE, mild vs. severe PE) further supported these findings, showing consistent or even more pronounced associations.

Our findings suggest that the genetic variant rs12415800 in SIRT1 may be linked to PE risk in Chinese Han women, with the AA genotype conferring a reduced risk. Further research is warranted to validate these findings in other populations and larger prospective studies.