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Culture and Electrophysiological Analysis of Patient-Specific iPSCs Using Microelectrode Array Dishes
Bayardo I Garay1, Rita C R Perlingeiro2
1Department of Medicine, Lillehei Heart Institute, University of Minnesota, Minneapolis, MN, USA.
Abstract:
Microelectrode arrays offer an exciting opportunity to probe and characterize the functional status of dystrophin-deficient muscle tissue vis-à-vis patient-specific iPSC-derived skeletal muscle (SkM) and cardiac muscle (CM). Here, we present a method to culture and analyze baseline electrophysiological profiles of Duchenne muscular dystrophy (DMD) iPSC-SkM and iPSC-CM using MEA dishes.
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