Related Experiment Video
Updated: Jan 16, 2026

05:53
Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
Published on: June 21, 2018
10.6K
Methods for the Construction of Core Collections Using SNP Markers
J Aravind1, Ankit Saroha2, Dhammaprakash Pandhari Wankhede3
1ICAR-National Bureau of Plant Genetic Resources, New Delhi, India. j.aravind@icar.org.in.
Methods in Molecular Biology (Clifton, N.J.)
|October 1, 2025
Summary
Core collections aid crop diversity management using genebanks. New methods leverage molecular data (SNPs) for optimized core set development, improving access to genetic resources.
Area of Science:
- Agricultural Science
- Genetics
- Bioinformatics
Background:
- Genebanks conserve vast crop germplasm collections.
- Core collections facilitate management and access to this diversity.
- Traditional methods relied on passport and phenotypic data.
Purpose of the Study:
- To explore advanced methods for constructing crop core collections.
- To demonstrate the utility of molecular marker data in core set development.
Main Methods:
- Utilizing single nucleotide polymorphism (SNP) data from high-throughput techniques.
- Applying optimization-based algorithms for core set construction.
- Illustrating software implementations like PowerCore, Core Hunter 3, coreCollection, and ShinyCore.
Main Results:
- Molecular data enables more efficient and accurate core set creation.
- Optimization algorithms effectively leverage SNP data for diversity capture.
- Software tools provide practical means for implementing these methods.
Conclusions:
- Modern molecular data significantly enhances the development of crop core collections.
- Leveraging SNP data and computational tools improves the representation of crop diversity.
- This approach optimizes the management and accessibility of valuable germplasm resources.
Related Concept Videos
Single Nucleotide Polymorphisms-SNPs
17.9K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
17.9K
Comparing Copy Number Variations and SNPs
18.6K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
18.6K
Sanger Sequencing
773.3K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
773.3K
Next-generation Sequencing
97.8K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
97.8K
DNA Microarrays
20.7K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
20.7K
RNA-seq
11.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.8K

