Agrobacterium-Mediated Genetic Transformation of Indica Rice
Rajadurai Gothandaraman1, Balaji Santhakumar1, Vignesh Ponnurangan1,2
1Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
Methods in Molecular Biology (Clifton, N.J.)
|October 1, 2025
Summary
This study presents an efficient genetic transformation protocol for indica rice using immature embryos. It highlights critical optimization points for improved rice functional genomics research.
Area of Science:
- Plant Science
- Genetics
- Biotechnology
Background:
- Functional genomics in rice is advancing due to high-quality genome data and gene characterization.
- Established rice transformation protocols exist but show cultivar-specific responses, necessitating optimization.
Purpose of the Study:
- To provide an efficient genetic transformation protocol for indica rice.
- To detail critical optimization points for rice immature embryo transformation.
Main Methods:
- Development and refinement of genetic transformation protocols.
- Focus on using indica rice immature embryos as explants.
Main Results:
- An efficient transformation protocol for indica rice has been established.
- Critical points for successful transformation using immature embryos are identified.
Conclusions:
- The developed protocol offers an optimized method for indica rice transformation.
- This advancement supports further functional genomics studies in rice.
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