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Transgenic Plants02:50

Transgenic Plants

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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Transgenic Organisms00:53

Transgenic Organisms

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Overview
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Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

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Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
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Trihybrid Crosses02:27

Trihybrid Crosses

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Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
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Related Experiment Video

Updated: Jan 16, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

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Characterization of Transgenic Rice Plants by Non-radioactive Southern Hybridization.

Rajasekaran Sripriya1, Mahitha Koonjangadan Padinjhareveedu2, Ajith Thaliyakkattil Girijan2

  • 1Department of Zoology, St. Mary's College, Thoothukudi, Tamil Nadu, India.

Methods in Molecular Biology (Clifton, N.J.)
|October 1, 2025
PubMed
Summary
This summary is machine-generated.

This study details a Southern hybridization protocol for detecting specific DNA sequences in rice plants. The method uses capillary transfer and a Digoxin-labeled probe for accurate gene analysis.

Keywords:
Alkaline phosphataseAnti-DIG antibodyBlockingCDP-star substrateCapillary transferChemiluminescenceDenaturationDepurinationNeutralizationPrehybridization

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Area of Science:

  • Molecular Biology
  • Genetics
  • Plant Science

Background:

  • Southern hybridization is a key technique for DNA sequence detection and gene analysis.
  • Accurate detection of specific DNA sequences is crucial in plant genetics research.

Purpose of the Study:

  • To describe a protocol for transgene detection in rice plants using Southern hybridization.
  • To optimize DNA transfer and hybridization for sensitive transgene identification.

Main Methods:

  • DNA digestion with restriction enzymes and electrophoresis.
  • Capillary transfer of DNA to a nylon membrane.
  • Hybridization with a Digoxin-labeled oligonucleotide probe and antibody detection.

Main Results:

  • Successful detection of specific DNA sequences in rice plants.
  • The protocol provides a reliable method for transgene identification.

Conclusions:

  • The described Southern hybridization protocol is effective for transgene detection in rice.
  • This method facilitates accurate gene analysis in genetically modified rice plants.