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Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

Transgenic Organisms

Transgenic Organisms

Plant Breeding and Biotechnology

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

Trihybrid Crosses

Trihybrid Crosses

Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...

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Related Experiment Video

Updated: Jan 16, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

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Characterization of Transgenic Rice Plants by Radioactive Northern Hybridization.

Rajasekaran Sripriya¹, Ajith Thaliyakkattil Girijan², Mahitha Koonjangadan Padinjhareveedu²

¹Department of Zoology, St. Mary's College, Thoothukudi, Tamil Nadu, India.

Methods in Molecular Biology (Clifton, N.J.)

|October 1, 2025

Summary

This summary is machine-generated.

Northern hybridization confirms transgene presence and expression in transgenic plants. This sensitive method visualizes transcript size and multiple forms using radioactive DNA probes.

Keywords:

Northern blotting RNA Radioactive Northern hybridization Rice

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Area of Science:

Molecular Biology
Plant Biotechnology
Genetics

Background:

Confirming transgene integration and expression is crucial in developing transgenic plants.
Northern hybridization is a well-established technique for RNA analysis.

Purpose of the Study:

To detail a protocol for Northern hybridization in transgenic rice.
To demonstrate the utility of Northern hybridization for confirming transgene-derived transcripts.

Main Methods:

Northern blotting and hybridization.
Radioactive labeling of DNA probes.
Analysis of RNA from transgenic rice plants.

Main Results:

Confirmation of transgene-derived transcript presence.
Determination of transcript size and relative expression levels.
Visualization of multiple transcript forms, if present.

Conclusions:

Northern hybridization is a sensitive and effective method for analyzing RNA in transgenic plants.
The described protocol facilitates the detection of specific RNA transcripts in rice.