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Updated: Jan 16, 2026
Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
Published on: June 11, 2010
Characterization of Transformed Plants by GUS Assay
Sankari Mohana1, Reshma Anjum Mohammed1, Golla Narasimha2
1Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh, India.
Abstract:
A reporter gene GUS (uid A) is a fusion protein used to study the expression pattern of transgenic plants. A gene fusion is a DNA structure (in vitro or in vivo) in which the regulatory sequences of one gene influence the transcription and/or translation of the coding sequences of another gene (a reporter). This histochemical assay is used to investigate the mechanism of plant transformation events, cellular expressions, function of the plant genome, and their level of expression in genes.
