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Updated: Aug 1, 2026

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Published on: February 25, 2010
A Guide to Fed-Batch Cultivation of Pichia pastoris on Laboratory Scale
Marcel Straumann1, Hana Raschmanová2, Valentina Haag3
1Molecular Partners AG, Schlieren, Switzerland. marcel.straumann@molecularpartners.ch.
Abstract:
Fed-batch is the most commonly used cultivation mode for industrial production of recombinant proteins with Pichia pastoris. On a laboratory scale, fed-batch culture provides a way to control the specific biomass growth rates at any pre-set value, allowing the conditions of biomass growth and recombinant product formation to be systematically studied.In this chapter, we present an accessible and versatile approach for designing, performing, and evaluating a fed-batch cultivation in laboratory-scale stirred tank bioreactors.
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