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Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
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Best Practices for Protein Expression in Pichia pastoris Combining Design of Experiments with an Automated Platform

Holger Morschett1, Joan Cortada-Garcia2, Hauke Holm2

  • 1Lonza AG, Visp, Switzerland. holger.morschett@lonza.com.

Methods in Molecular Biology (Clifton, N.J.)
|October 1, 2025
PubMed
Abstract

Keywords:
AutomationDesign of experimentsFed-batchKomagataella phaffiiOptimizationParallelized cultivationPichia pastorisProcess developmentSingle-use

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