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Updated: Jan 16, 2026

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris

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Published on: February 25, 2010

Pichia pastoris Cell Disruption by High-Pressure Homogenization (HPH).

Antoni Casablancas¹, Xavier Garcia-Ortega¹, Francisco Valero²

¹Department of Chemical, Biological and Environmental Engineering, Engineering School, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.

Methods in Molecular Biology (Clifton, N.J.)

|October 1, 2025

Summary

High-pressure homogenization (HPH) efficiently disrupts cells for product recovery. This guide details applying HPH to the Komagataella phaffii yeast cell factory for improved downstream processing.

Keywords:

Cell disruption Critical process parameter (CPP)High-pressure homogenization Key performance indicators (KPI)Komagataella phaffii Pichia pastoris

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Last Updated: Jan 16, 2026

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris

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Published on: October 30, 2013

Area of Science:

Biotechnology
Bioprocess Engineering
Cell Biology

Background:

Cell disruption is crucial for recovering intracellular products.
Various methods exist, but high-pressure homogenization (HPH) offers efficiency and scalability.
Komagataella phaffii is a significant yeast host for recombinant protein production.

Purpose of the Study:

To provide a comprehensive guide for applying high-pressure homogenization (HPH) to the yeast Komagataella phaffii.
To detail the methodology for effective cell disruption in this specific microbial system.
To optimize the recovery of intracellular products from K. phaffii.

Main Methods:

High-pressure homogenization (HPH) as the primary cell disruption technique.
Focus on the application and optimization of HPH parameters for K. phaffii.
Downstream processing considerations for liberated intracellular products.

Main Results:

Demonstration of HPH as an effective method for disrupting K. phaffii cells.
Characterization of cell disruption efficiency based on HPH parameters.
Successful release of intracellular target products from K. phaffii.

Conclusions:

High-pressure homogenization is a highly effective and scalable method for disrupting Komagataella phaffii.
This guide facilitates the implementation of HPH for enhanced intracellular product recovery in K. phaffii.
Optimized HPH protocols are essential for efficient bioprocessing using K. phaffii.