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Updated: Jan 16, 2026
Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
Published on: January 23, 2019
Determination of Biomass Elemental Composition of Pichia pastoris
Arnau Gasset1, Francisco Valero1, José Luis Montesinos-Seguí2
1Department of Chemical, Biological and Environmental Engineering, School of Engineering, Universitat Autònoma de Barcelona, Bellaterra (Barcelona), Spain.
Abstract:
A quantitative determination of a consistent biomass elemental composition is required for a wide range of applications, including metabolic engineering, bioprocess parameter calculation, and medium optimization, among others. Its determination relies on the use of different analytical techniques for the range of biochemical compounds considered. In this chapter, a robust methodology is proposed for its determination of yeast, specifically Komagataella phaffii (previously named Pichia pastoris).
