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Oligo-dT Anchored cDNA SCoT: An Innovative Approach for Deciphering Fusarium Wilt Resistance-Related Differential
S L Abhang1, P Sharma1,2, S N Datarkar1
1Biotechnology Centre, Dr. PDKV, Akola, Maharashtra, India.
None:
The cDNA start codon targeted marker (cDNA-SCoT) methodology is a unique, simple, quick, and efficient method for investigating differential gene expression. The differentially expressed fragments (DEFs) in resistant and susceptible cultivars were identified using cDNA-SCoT profiling. Sanger sequencing and the nBLAST homology search program were used to characterize the functional properties of DEFs. As a result, this methodology is innovative and would be utilized in future studies to identify potential functional genes, which can then be used in marker creation, marker-assisted selection, and uncovering the regulatory role of the relevant genes.
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