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Updated: Jan 16, 2026
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Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse
Published on: August 15, 2018
14.2K
Rodent Enteric Glia Culture.
Jacques Gonzales1, Tony Durand2, Brian D Gulbransen3
1Department of Physiology, Michigan State University, East Lansing, MI, USA. gonza984@msu.edu.
Methods in Molecular Biology (Clifton, N.J.)
|October 1, 2025
Summary
This study presents a reliable method for culturing pure enteric glia, essential cells for gut health. This technique aids in understanding their role in intestinal function and disease.
Area of Science:
- Neuroscience
- Gastroenterology
- Cell Biology
Background:
- Enteric glia are crucial for maintaining the enteric nervous system's plasticity and homeostasis.
- Dysfunctional enteric glia are linked to intestinal disorders and gut pathophysiology.
- A pure primary culture method is needed to study enteric glia in gut physiology.
Purpose of the Study:
- To establish a protocol for isolating, purifying, enriching, and culturing pure enteric glia from mature rodent intestines.
- To provide a reliable tool for researchers investigating the role of enteric glia in gut function.
Main Methods:
- Isolation of cells from mature rodent intestines.
- Purification and enrichment techniques to obtain a pure glial population.
- Establishment of a primary cell culture protocol.
Main Results:
- Successful isolation and purification of enteric glia.
- Development of a reproducible primary culture method.
- Characterization of cultured enteric glia (data not shown in abstract).
Conclusions:
- The described protocol yields pure and reliable primary cultures of enteric glia.
- This method facilitates further research into enteric glial function in gut physiology and disease.
- Provides a foundation for studying enteric glia in various gastrointestinal contexts.
