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Related Experiment Video

Updated: Jan 16, 2026

Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse
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Rodent Enteric Glia Culture.

Jacques Gonzales1, Tony Durand2, Brian D Gulbransen3

  • 1Department of Physiology, Michigan State University, East Lansing, MI, USA. gonza984@msu.edu.

Methods in Molecular Biology (Clifton, N.J.)
|October 1, 2025
PubMed
Summary

This study presents a reliable method for culturing pure enteric glia, essential cells for gut health. This technique aids in understanding their role in intestinal function and disease.

Keywords:
AdultCell cultureEnteric gliaGliaIsolationMousePurificationRat

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Area of Science:

  • Neuroscience
  • Gastroenterology
  • Cell Biology

Background:

  • Enteric glia are crucial for maintaining the enteric nervous system's plasticity and homeostasis.
  • Dysfunctional enteric glia are linked to intestinal disorders and gut pathophysiology.
  • A pure primary culture method is needed to study enteric glia in gut physiology.

Purpose of the Study:

  • To establish a protocol for isolating, purifying, enriching, and culturing pure enteric glia from mature rodent intestines.
  • To provide a reliable tool for researchers investigating the role of enteric glia in gut function.

Main Methods:

  • Isolation of cells from mature rodent intestines.
  • Purification and enrichment techniques to obtain a pure glial population.
  • Establishment of a primary cell culture protocol.

Main Results:

  • Successful isolation and purification of enteric glia.
  • Development of a reproducible primary culture method.
  • Characterization of cultured enteric glia (data not shown in abstract).

Conclusions:

  • The described protocol yields pure and reliable primary cultures of enteric glia.
  • This method facilitates further research into enteric glial function in gut physiology and disease.
  • Provides a foundation for studying enteric glia in various gastrointestinal contexts.