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Updated: Jan 16, 2026

Innervation of Human Intestinal Organoids
Published on: January 17, 2025
Differentiating Enteric Glial Cells Within Intestinal Organoids Derived from Human Pluripotent Stem Cells
Simon Vales1, Lola Bonneau2, Maxime M Mahe3,4,5
1Université de Nantes, Inserm, TENS, The Enteric Nervous System in Gut and Brain Diseases, IMAD, Nantes, France.
None:
Human intestinal organoids (HIOs) derived from pluripotent stem cells are valuable tools for studying gastrointestinal development and function. Herein, we present a method for generating and co-culturing human intestinal organoids and enteric glial cells derived from human pluripotent stem cells. In addition, we describe how to collect and stain enteric glial cells within organoids embedded in Matrigel. The method outlines the precise steps required to successfully generate both human intestinal organoids and vagal neural crest cells, offering valuable insight into the study of human enteric glial cells.
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